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These alterations are the probable result of the lengthy cellular damage induced by MTX resulting in increased levels of ROS leading to DNA injury, oxidation, and proliferation inhibition

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[25] The use of integrated multi-omics techniques RNA-seq, assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) and chromatin Immunoprecipitation sequencing (ChIP-seq) enables researchers to further identify epigenetic elements, key interacting genes N-acetyltransferase 1 (NAT1), Pre-B-cell leukemia homeobox transcription factor 1 (PBX1), RNA recognition motif (RRM)