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10.1590/18069061-2015-0155 151 KawaharaK.HohjohH.InazumiT.TsuchiyaS.SugimotoY

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HAP1 cells with a knockout of mitochondrial trans-2-enoyl-CoA reductase, an enzyme required for lipoic acid (LA) synthesis, were used as a positive control because the majority of DLAT is expected to be unlipoylated in these cells (confirmed by western blot in Supplementary Fig