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3) The general procedure for studying the effect of the pH on the rate of SetCys to Cys conversion is illustrated with the following conditions: 1 mM peptide, 200 mM MPAA, 100 mM TCEP, 100 mM ascorbate, pH 6.90 in 0.1 M phosphate buffer

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Size:6 x 10mg/10mg

TLGST was inhibited with iodoacetamide indicated that residues of cysteine, histidine and methionine have significant impacts on structure and effectiveness of TLGST