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Avoid repeated freeze-thaw cycles, which reduce recovery of the 43-residue thymosin beta 4 chain in particular, label every aliquot with the reconstitution date, and handle throughout under sterile laboratory conditions

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Complications: 7 patients (54%) developed acute GVHD: 6 (46%) grade I/II and 1 (8%) grade IV, after a median of 26 days (21-48)