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Cytotoxicity Evaluation of Chlorambucil-Platinum(IV) Prodrugs in Colorectal Cancer Cells, Normal Epthelial Cells and Spheroid Model of Colorectal Cancer The cytotoxic effect of the CLB-platinum(IV) prodrugs was examined in HT29 colon cancer cells and spheroids and MCF10A non-tumourigenic immortalised human breast epithelial cells 72 h post-treatment (Figure 1 and Table 1)