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Centrifugation-cleared lysate was applied to Glutathione Sepharose 4B (GE Healthcare) and washed with 20 mM Tris-HCl (pH 8.0), 300 mM NaCl, 0.01% NP-40, 10% glycerol, 1 mM DTT, 1 μg/ml benzamidine

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Nitrogen, sulfur, and phosphorus co-doped carbon dots-based ratiometric chemosensor for highly selective sequential detection of Al 3+ and Fe 3+ ions in logic gate, cell imaging, and real sample analysis