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Hearts were perfused with EDTA buffer (130 mM NaCl, 5 mM KCl, 0.5 mM NaH 2 PO 4 , 10 mM HEPES, 10 mM glucose-monohydrate, 10 mM taurine and 5 mM EDTA, pH 7.8) for 2 min inside the animal and then quickly excised and perfused for an additional 6 min to clear all the blood from the hearts