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Pharmacokinetic information is not available as to whether or not N-acetylcysteine crosses the blood-brain barrier or placenta, or into breast milk

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The cells were collected, washed twice with cold PBS and resuspended in immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.5, 5 mM EDTA, 0.5% NP-40 and 1% Triton X-100) for ultrasound fragmentation