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In situ Staining of Diaminobenzidine (DAB) and Nitroblue Tetrazolium (NBT) For detection of H 2 O 2 , paraquat or water treated Arabidopsis seedlings were vacuum infiltrated with 0.1% 3,3′-Diaminobenzidine (DAB) in 10 mM MES pH 6.5 solution, washed three times with water and incubated for 45 min at RT in light