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After incubation, samples were placed on ice, washed with BSST (BSS + 0.05% Tween 20), and residual folded p/p*MHCs were detected with PE anti-human β2m antibody (clone 2M2, 1:500, BioLegend, 316305)

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Peptides improve synovial environment : Reduce inflammatory cytokines in joint fluid Enhance nutrient delivery Support healthy synovial membrane Result : Better joint function, less grinding, improved mobility