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2.2 Drugs and chemicals We purchased MTX (MYLAN) from Haupt Pharma GmbH, Germany), NTZ was purchased from Medizen Pharmaceutical Ind (Alexandria, Egypt), N-acetylcysteine (NAC) was obtained from SEDICO (Giza, Egypt), whereas Sigma–Aldrich company (MO, United States) provided Kolliphor ® EL and 4-Phenylbutyric acid (4-PBA)

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It is localized in mitochondria of several tissues, including skeletal muscle, where it regulates O 2 consumption [34]