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Therefore, we suggested that TNF- α increase by IS induced ROS overproduction in the kidney, which is involved in kidney fibrosis

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Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 μl mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added