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Glutathione Production from Spent Yeast of Bioethanol Manufacture: Impact of Extraction Techniques with Commercial Applicability of Biotechnological Significance | Topics in Catalysis | Springer Nature Link

They require an initial step of thiol alkylation of free unreactive cysteines using generally N-ethylmaleimide (NEM), iodoacetamide (IAM) for irreversible modifications or MMTS (methyl methanethiosulfonate) for reversible modifications

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Only the substrate orientation (Fig