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Size (mL):10mL

Assuming 1 ) an average ASL height of 5 μm on the top of epithelial cells grown on 0.33 cm 2 of a membrane insert and 2 ) an ASL osmolarity of 305 mOsm/kg, the osmolarities of the test compounds were mildly diluted (∼5%) upon their addition (1 μl) to the mucus layer of the cell culture based on resident ASL