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Receptor functionality is often confirmed by the binding of a specific ligand, monitored via interaction assays

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Inhibiting key glycolytic enzymes (Oxamate, 2-DG, Shikonin and Simvastatin) or disrupting lactate transport via monocarboxylate transporters (MCTs), e.g., using Syrosingopine to inhibit MCT4, can effectively deplete lactate, the fundamental driver of lactylation (60,65,103)