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New primers were designed by adding the sequence of promoter T7 (5′-TAA TAC GAC TCA CTA TAG GG-3′) to the 5′ portion of each original gene specific primer sequence (Table S4) and they were used to synthesize dsRNA from the P

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The alterations in bile acid metabolizing bacteria were accompanied by significant alterations in the serum concentration of secondary bile acids, including a marked depletion of α-muricholic acid