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The United States Pharmacopeia (USP) XXII assay for hyaluronidase determines hyaluronidase activity indirectly by measuring the amount of higher molecular weight hyaluronic acid, or hyaluronan, (HA) substrate remaining after the enzyme is allowed to react with the HA for 30 min at 37 0 C (USP XXII-NF XVII ( 1990) 644-645 United States Pharmacopeia Convention, Inc, Rockville, MD)

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