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After 18 h of incubation with 100 μ M BSO or 25 mM NAC, the intracellular GSH levels were quantified using the GSH-Glo GSH Assay (Promega), as recommended by the manufacturer's instructions

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This observation in older adults is consistent with previous studies that report associations of low glycine status with elevated insulin resistance ( We next asked whether 2 weeks of daily supplementation with GlyNAC increased fasting levels of glycine towards that of young subjects