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For recombinant protein expression, High Five cells grown in SF-4 baculo express insect medium (BioConcept) at a density of 2.0 × 10 6 cells per ml were infected with baculovirus at 1.5% v/v ratio

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These findings support the dominant role of cystine uptake in maintaining tumor growth, and suggest that de novo cysteine synthesis cannot fully compensate for loss of exogenous cyst(e)ine in some cancers