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In the N-terminal tagged configuration, deleting the stalk region resulted in a significant loss of blocking by C34, C37 and CHK-263 mAbs while no significant Mxra8 binding ratio change by a fusion loop targeting neutralizing antibody SKT20 56 , indicating the existence of interaction between the above three mAbs with stalk region of CHIKV E, and this interaction contribute to blocking of Mxra8 binding.,