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Crystal drops were prepared by adding 150 nL of 28.6 mg/mL TRF1 TRFH to 150 nL of reservoir solution (100 mM MES pH 6.0, 35–45% PEG200, 50 mM CaCl 2 ), against 35 µL reservoir, and reached their maximum size (200–300 μm across) after 5 d