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Best crystals grew in 50 mM HEPES, pH 7.0, 4% PEG 8000, 100 mM NaCl and were cryoprotected by transferring to a solution containing 25% glycerol as cryoprotectant along with all the components of protein and crystallization mother liquor

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The ratio of GSH to GSSG can be changed by a number of factors including disease state or oxidative stressors

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