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The rate of decrease of absorbance at 340 nm is directly proportional to LDH or SDH activity in the sample, where one unit of LDH or SDH activity is defined as 0.1 μmol of substrate transformed/minute at pH 7.6 at 25°C.The SDH enzymatic activity was determined as the amount of fructose reacted per unit of time, and it was measured by recording the absorbance decrease due to NADH oxidation

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