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Mitophagy and diabetic microvascular complications Mitophagy and DKD Molecular mechanisms of mitophagy in DKD In high glucose (HG)-induced human podocytes and Zucker rat models, mitophagy was found to be inhibited, albumin leakage was increased in a hyperglycemic environment, and it was found that PINK1 plays an important role in insulin signaling and maintenance of podocyte permeability and that PINK1 possibly termed as a potential target for the treatment or prevention of DKD [110]

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Before incubation with molecules, the cells were washed twice with PBS and then incubated with 10 µM molecules for 1 hour in one of three conditions – medium with FBS, medium without FBS, and PBS