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43 The use of the anti-αvβ3 antibody significantly downregulated cell spreading but the use of the anti-β1 antibody and anti-α5 antibody upregulated cell spreading

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The cells (3 × 10 6 cells/mL) were transfected with 1 µg of plasmid DNA per mL of cell culture using ExpiFectamine 293 Reagent (Gibco)