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Differences between groups were statistically analyzed using one-way analysis of variance and Tukey's post-hoc test

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We followed the response of two OVA-expressing tumor cells, namely the breast cancer line E0771 and the melanoma cancer line B16, introduced into immunocompetent C57BL/6 recipient mice to intravenously injected CTLs derived from the CD8 T cell OT-I transgene ( in vivo killing activity of intravenously injected OT-I CTLs towards primary OVA-expressing E0771 breast tumors orthotopically implanted in mammary fat pads