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Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby specifically inhibits ferroptosis

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Cell viability was tested by the Cell Counting Kit-8 (CCK-8, Dojindo) that is based on the reduction of the water-soluble tetrazolium salt WST-8 [2-(2-methoxy-4-nitrophenyl)−3-(4-nitrophenyl)−5-(2,4-disulfophenyl)−2 H-tetrazolium, monosodium salt] by cellular dehydrogenases, producing a water-soluble orange formazan dye