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Topical hydrocortisone also has an anti-mitotic effect by decreasing epidermal mitosis mediated through an increase in lipocortin, an endogenous glucocorticoid-regulated protein

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Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark