5-amino-1mq nnmt inhibitor snp Australia 10mg Lot #:Lot #: N95A1MQ10/0428 Methylcobalamin B12 Injections UK Molecular Biology

5-amino-1mq nnmt inhibitor snp Australia 10mg Lot #:Lot #: N95A1MQ10/0428 Methylcobalamin B12 Injections UK Molecular Biology

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Molecular Biology

Lot #:Lot #: N95A1MQ10/0428

Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich