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Then, FUS proteins bound to the resin were washed with wash buffer (6 M GdnHCl, 50 mM Tris, and 1 M NaCl, pH 7.0), and eluted with elution buffer (6 M GdnHCl, 50 mM Tris, 100 mM NaCl, and 250 mM imidazole, pH 7.0)

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Rogers and Munné-Bosch, 2016), further research on fruit ROS is necessary to unveil the actual ROS-generating compartments and processes that mostly contribute to ROS production in fruit tissues