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You need two different needles for the process: Reconstitution (drawing + mixing): 18–22 gauge, 1–1.5 inch needle - larger bore makes it easy to draw up BAC water without excessive pressure Subcutaneous injection: 27–31 gauge, ½ inch needle - thin needle minimizes pain and is appropriate for shallow SubQ deposits Intramuscular injection: 25–27 gauge, 1–1.5 inch - longer needle reaches muscle tissue Many people use a drawing needle to pull from the vial, then swap to a fresh injection needle

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Then, we successfully cloned and expressed the SmGST recombinant protein and studied its enzymatic characteristics