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Western blotting analysis of complex I and PGC-1α protein expression Protein expression of complex I and PGC-1α was detected using Western blotting with primary antibodies directed against PGC-1α (1:1000) or complex I (1:2000) and with horseradish peroxidase-conjugated secondary antibody

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DNMT3L has an accessory function to DNMT3A and DNMT3B de novo methylation and, in humans, is expressed only in germ cells and embryonic stem cells but not in somatic cells