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To stain for collagen, sections were incubated for 60 minutes in Picro Sirius Red solution (1g/L in picric acid) and briefly rinsed twice in 0.05% acetic acid

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(major emphasis is Phase I metabolism – very thorough coverage) Smith, Glucuronidation, UNC Chapel Hill, 9/2011 Conjugation Reactions – Some Definitions Glucuronidation “conjugation” is the addition of a molecule to the drug or xenobiotic GSH conjugation GSH = glutathione (a tripeptide derivative) Sulfate conjugation Amino acid conjugation Acetylation Methylation “Phase II metabolism” is terminology coined by RT Williams, whereby a compound is first subject to oxidation reduction or hydrolysis (Phase I reactions), which may be associated with bioactivation, and then the functional group created is conjugated to a less toxic or inactive compound