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RNP was allowed to form in P3 solution for at least 5 minutes at room temperature, and the nucleofection mixture was loaded into the 16-well nucleocuvette with the total volume less than 25 μL

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In summary, changes in oxidative stress parameters in RA include both increased levels of oxidative damage markers such as MDA, 8-OHdG, and AOPPs, as well as the activation of the AGE-RAGE pathway, which drives inflammatory processes, and the decreased activity of antioxidant enzymes such as SOD, CAT, and GPx

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