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2.17 Safety assessment distribution and biological After treatment, nude mice were killed by cervical dislocation, and tumors and major organs (heart, liver, spleen, lung, and kidney) of the respective group were extracted, fixed with 4% paraformaldehyde solution, embedded in paraffin, staining with hematoxylin and eosin (H&E), and imaging with digital microscope (HAMAMATSU S210 microscope)