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For each sample, 200 µl of supernatant was added to 600 µl of thiobarbituric acid (TBA) solution, reconstituted in glacial acetic acid, and then incubated at 95 °C for 60 min in a dry-block thermostat (Bio TDB-100, Biosan, Latvia) and finally cooled immediately on ice for 10 min

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A smart single-loop-mediated isothermal amplification facilitates flexible SNP probe design for on-site rapid differentiation of SARS-CoV-2 omicron variants