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Empirical MinION™ data confirmed these predictions: full-length operon sequencing identified 100% of target species with fewer misclassifications, outperforming both V3–V4 (80% correct) and single 16S (∼95%), thus highlighting the combined power of 18S rRNA/ITS region (rRNA operon) for accurate microbiome profiling (Olivier et al., 2023)