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Aside from histological methods, TJPs can be estimated by assessing the protein expression using the Western blot technique, as documented in a human trial of patients with intestinal barrier abnormalities [48], or by evaluating the mRNA levels of TJPs by RT-qPCR, as has been assayed in colonic tissues from healthy human volunteers [49]

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With the subcutaneous route of administration, the initial recommended dose is 1.0 to 5.0 µg once or twice daily, depending on the size of the animals