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374 In general, the expression of phage libraries containing ncAAs depends on a three-plasmid expression system including one plasmid encoding randomized peptide library, one plasmid encoding the RS/tRNA pair essential for amber suppression using a ncAA and another helper phage plasmid which supplies the necessary phage proteins for packaging

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Extended storage of reconstituted solution: For storage beyond 30 days, prepare sterile aliquots and freeze at −20 °C (−4 °F)